Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
nm23-H2 CRISPR/Cas9 KO Plasmid (h): sc-416164
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- nm23-H2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the nm23-H2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: nm23-H2 Antibody (X-42): sc-100400
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
nm23-H2 CRISPR/Cas9 KO Plasmid (h) | sc-416164 | 20 µg | $397.00 |
Overview
NME1-NME2 encodes nm23-H2, a nucleoside diphosphate kinase that helps maintain cellular nucleotide homeostasis by catalyzing phosphate transfer between NTPs and NDPs. Beyond its metabolic role, nm23-H2 participates in regulation of gene expression and signaling networks linked to proliferation, differentiation, and stress responses, with reported functions in nucleic acid binding and transcriptional control in the nucleus. Altered NME1/NME2 activity and expression patterns have been associated with tumor progression and metastatic behavior in multiple cancer contexts, making it a useful node for mechanistic studies of invasion and cell-state transitions. In human cells, nm23-H2 is also studied in relation to cytoskeletal remodeling and motility pathways that influence migration phenotypes.
nm23-H2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NME1-NME2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NME1-NME2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NME1-NME2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish nm23-H2 protein expression.
This CRISPR knockout system enables efficient generation of NME1-NME2-deficient cell models for investigation of nm23-H2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NME1-NME2 exon(s) critical for nm23-H2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NME1-NME2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by nm23-H2 CRISPR/Cas9 KO Plasmid (h) and nm23-H2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NME1-NME2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by nm23-H2 HDR Plasmid (h) and nm23-H2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NME1-NME2 homology arms to support homology-directed repair at defined NME1-NME2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.