neuroserpin Double Nickase Plasmid (m): sc-423113-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- neuroserpin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- neuroserpin Double Nickase Plasmid (m) and neuroserpin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Serpini1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: neuroserpin Antibody (C-9): sc-48360
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
neuroserpin Double Nickase Plasmid (m) | sc-423113-NIC | 20 µg | $410.00 | |||
neuroserpin Double Nickase Plasmid (m2) | sc-423113-NIC-2 | 20 µg | $410.00 |
Mouse Serpini1 encodes neuroserpin, a secreted serine protease inhibitor that primarily modulates tissue-type plasminogen activator (tPA/PLAT) activity in the nervous system. By restraining pericellular proteolysis, neuroserpin influences extracellular matrix remodeling, synaptic plasticity, neurite outgrowth, and activity-dependent signaling pathways linked to neuronal survival and circuit refinement. Serpini1 is also implicated in cellular proteostasis, as misfolding or altered expression can perturb ER stress responses and protein quality control. Dysregulation of neuroserpin-tPA/plasmin axis has been associated with neurodegenerative and neuroinflammatory mechanisms, making Serpini1 a relevant target for studying pathways that couple proteolysis to neuronal function.
neuroserpin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Serpini1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Serpini1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Serpini1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Serpini1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.