NDST1 Double Nickase Plasmid (h): sc-404078-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NDST1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NDST1 Double Nickase Plasmid (h) and NDST1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NDST1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NDST1 Antibody (FF-2): sc-100790
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NDST1 Double Nickase Plasmid (h) | sc-404078-NIC | 20 µg | $410.00 | |||
NDST1 Double Nickase Plasmid (h2) | sc-404078-NIC-2 | 20 µg | $410.00 |
NDST1 (N-deacetylase/N-sulfotransferase 1) is a key Golgi-resident enzyme that catalyzes N-deacetylation and N-sulfation steps during heparan sulfate biosynthesis, shaping the sulfation patterns that determine glycosaminoglycan–protein interactions. By controlling heparan sulfate domain structure, NDST1 modulates extracellular matrix organization and ligand availability for pathways such as FGF, WNT, BMP, and Hedgehog signaling, influencing cell adhesion, migration, and morphogen gradient formation. Altered NDST1 activity and heparan sulfate sulfation have been linked to dysregulated developmental programs and disease-associated remodeling of cell–matrix signaling. As a result, NDST1 is widely studied for its role in proteoglycan-mediated regulation of growth factor signaling and tissue homeostasis.
NDST1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NDST1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NDST1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NDST1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NDST1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.