NaDC-3 Double Nickase Plasmid (h): sc-416648-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NaDC-3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NaDC-3 Double Nickase Plasmid (h) and NaDC-3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC13A3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NaDC-3 Antibody (3A6): sc-293347
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NaDC-3 Double Nickase Plasmid (h) | sc-416648-NIC | 20 µg | $410.00 | |||
NaDC-3 Double Nickase Plasmid (h2) | sc-416648-NIC-2 | 20 µg | $410.00 |
SLC13A3 encodes the human NaDC-3 sodium-dependent dicarboxylate transporter (SLC13 family), a plasma membrane carrier that couples the Na⁺ gradient to uptake of Krebs cycle intermediates such as succinate, α-ketoglutarate, and citrate. By regulating intracellular availability of dicarboxylates, NaDC-3 supports central carbon metabolism, anaplerosis, and redox balance, and can influence cellular responses to metabolic stress. The transporter is prominently studied in renal and hepatic physiology where organic anion handling and energy metabolism intersect, and altered SLC13A3 activity has been investigated in contexts of metabolic dysregulation and organ injury models. As a gatekeeper for dicarboxylate flux, NaDC-3 is also relevant to pathways linking metabolite transport to signaling, including succinate-associated inflammatory and hypoxia-adaptive processes.
NaDC-3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC13A3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC13A3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC13A3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC13A3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.