Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MKP-1/DUSP1 CRISPR/Cas9 KO Plasmid (m): sc-422507
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MKP-1/DUSP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MKP-1/DUSP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MKP-1/DUSP1 Antibody (E-6): sc-373841
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MKP-1/DUSP1 CRISPR/Cas9 KO Plasmid (m) | sc-422507 | 20 µg | $397.00 |
Overview
Dual specificity phosphatase 1 (Dusp1), also known as MKP-1/DUSP1, is an inducible MAPK phosphatase that dephosphorylates and inactivates ERK1/2, JNK, and p38 MAPKs to constrain signal duration after stress, cytokine, and growth factor stimulation. In mouse cells, DUSP1 functions as a key feedback regulator linking MAPK activity to transcriptional programs governing inflammation, apoptosis, differentiation, and adaptation to oxidative and genotoxic stress. Through modulation of MAPK-dependent transcription factors and downstream cytokine outputs, DUSP1 influences innate immune signaling and cellular stress responses. Dysregulated DUSP1 expression or activity has been associated with altered inflammatory phenotypes and aberrant MAPK signaling in contexts including metabolic and oncogenic stress, supporting its use as a mechanistic node for pathway interrogation.
MKP-1/DUSP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dusp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dusp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dusp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MKP-1/DUSP1 protein expression.
This CRISPR knockout system enables efficient generation of Dusp1-deficient cell models for investigation of MKP-1/DUSP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dusp1 exon(s) critical for MKP-1/DUSP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dusp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MKP-1/DUSP1 CRISPR/Cas9 KO Plasmid (m) and MKP-1/DUSP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dusp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MKP-1/DUSP1 HDR Plasmid (m) and MKP-1/DUSP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dusp1 homology arms to support homology-directed repair at defined Dusp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.