Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Mitofilin Double Nickase Plasmid (h): sc-403617-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mitofilin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mitofilin Double Nickase Plasmid (h) and Mitofilin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IMMT. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mitofilin Antibody (D-3): sc-390707
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mitofilin Double Nickase Plasmid (h) | sc-403617-NIC | 20 µg | $410.00 |
IMMT encodes mitofilin, an essential inner mitochondrial membrane protein and core component of the MICOS complex that organizes cristae junctions and maintains mitochondrial ultrastructure. By shaping cristae architecture, mitofilin supports oxidative phosphorylation efficiency, metabolite diffusion, and proper assembly of respiratory chain supercomplexes, linking IMMT to bioenergetic homeostasis and mitochondrial quality control. IMMT-dependent membrane organization interfaces with mitochondrial dynamics and stress signaling, influencing processes such as apoptosis and mitophagy. Dysregulation of cristae maintenance and MICOS function is frequently studied in contexts of mitochondrial dysfunction observed across neurodegeneration, cardiometabolic stress, and cancer cell metabolism.
Mitofilin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IMMT locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IMMT. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IMMT function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IMMT-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.