Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MISR II Double Nickase Plasmid (h): sc-402523-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MISR II Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MISR II Double Nickase Plasmid (h) and MISR II Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AMHR2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MISR II Antibody (D-9): sc-377413
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MISR II Double Nickase Plasmid (h) | sc-402523-NIC | 20 µg | $410.00 | |||
MISR II Double Nickase Plasmid (h2) | sc-402523-NIC-2 | 20 µg | $410.00 |
AMHR2 (MISR II) encodes the anti‑Müllerian hormone type II receptor, a transmembrane serine/threonine kinase that initiates signaling in response to AMH. Upon ligand binding, MISR II recruits type I receptors and activates SMAD1/5/8 phosphorylation with downstream transcriptional programs that regulate Müllerian duct regression and broader reproductive tissue differentiation. In human cells, AMHR2 activity intersects with developmental and endocrine pathways that shape gonadal function and reproductive tract biology. Dysregulated AMHR2 expression or signaling has been associated with disorders of sex development and has been studied in the context of ovarian granulosa cell tumors and other reproductive system pathologies.
MISR II Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AMHR2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AMHR2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AMHR2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AMHR2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.