Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Menin Double Nickase Plasmid (m): sc-421626-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Menin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Menin Double Nickase Plasmid (m) and Menin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Men1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Menin Antibody (B-9): sc-374371
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Menin Double Nickase Plasmid (m) | sc-421626-NIC | 20 µg | $410.00 | |||
Menin Double Nickase Plasmid (m2) | sc-421626-NIC-2 | 20 µg | $410.00 |
Mouse Men1 encodes menin, a predominantly nuclear scaffold protein that integrates transcriptional control with chromatin regulation. Menin associates with histone-modifying complexes, including MLL/COMPASS family H3K4 methyltransferases, to modulate gene programs involved in cell-cycle control, lineage specification, and endocrine tissue homeostasis. Through interactions with transcription factors and DNA repair-associated proteins, menin influences replication stress responses and genome stability. Disruption of MEN1 function is linked to endocrine neoplasia biology and altered transcriptional networks, making Men1 a key node for modeling tumor suppressor pathways in murine systems.
Menin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Men1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Men1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Men1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Men1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.