MDC1 Double Nickase Plasmid (h): sc-405652-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MDC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MDC1 Double Nickase Plasmid (h) and MDC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MDC1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MDC1 Double Nickase Plasmid (h) | sc-405652-NIC | 20 µg | $410.00 | |||
MDC1 Double Nickase Plasmid (h2) | sc-405652-NIC-2 | 20 µg | $410.00 |
Mediator of DNA damage checkpoint 1 (MDC1) is a chromatin-associated scaffold protein that amplifies DNA double-strand break signaling by binding phosphorylated H2AX (γH2AX) and coordinating recruitment of repair and checkpoint factors. Through interactions with ATM-dependent phosphorylation events and ubiquitin signaling modules, MDC1 supports assembly of 53BP1 and BRCA1 pathway components, influencing pathway choice between non-homologous end joining and homologous recombination. MDC1 function is central to S-phase and G2/M checkpoint control, replication stress responses, and maintenance of genome stability. Altered MDC1 signaling has been associated with genomic instability phenotypes that are frequently observed in cancer biology and other DNA repair–related disorders.
MDC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MDC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MDC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MDC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MDC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.