MCM3AP CRISPR/Cas9 KO Plasmid (h): sc-406369
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MCM3AP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MCM3AP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCM3AP CRISPR/Cas9 KO Plasmid (h) | sc-406369 | 20 µg | $397.00 |
Overview
MCM3AP (MCM3-associated protein) is a multifunctional nuclear factor that interacts with the MCM2–7 helicase complex and contributes to regulation of DNA replication and cell-cycle progression. It has been linked to processes that coordinate replication licensing with transcriptional control and RNA metabolism, reflecting roles in genome maintenance and proliferative capacity. Disruption or altered expression of MCM3AP has been investigated in the context of replication stress, chromatin-associated signaling, and cellular responses to DNA damage that influence genomic stability. These mechanisms are frequently interrogated in cancer biology and other proliferative disorders where replication–transcription conflicts and checkpoint dysfunction are prominent research themes.
MCM3AP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MCM3AP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MCM3AP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MCM3AP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MCM3AP protein expression.
This CRISPR knockout system enables efficient generation of MCM3AP-deficient cell models for investigation of MCM3AP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MCM3AP exon(s) critical for MCM3AP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MCM3AP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MCM3AP CRISPR/Cas9 KO Plasmid (h) and MCM3AP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MCM3AP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MCM3AP HDR Plasmid (h) and MCM3AP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MCM3AP homology arms to support homology-directed repair at defined MCM3AP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.