Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Mast Cell Tryptase CRISPR/Cas9 KO Plasmid (h): sc-400887
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mast Cell Tryptase CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Mast Cell Tryptase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mast Cell Tryptase Antibody (G3): sc-33676
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mast Cell Tryptase CRISPR/Cas9 KO Plasmid (h) | sc-400887 | 20 µg | $397.00 |
Overview
TPSAB1 encodes mast cell tryptase, a secreted serine protease stored in cytoplasmic granules and released during mast cell degranulation. Mast cell tryptase contributes to proteolytic remodeling of the extracellular matrix, modulation of cytokine and chemokine signaling, and activation of protease-activated receptors, shaping inflammatory and tissue-repair programs. Its activity is linked to mast cell–driven processes such as vascular permeability, leukocyte recruitment, and airway reactivity, with relevance to allergic inflammation and other immune-mediated disorders. As a granule-associated protease, mast cell tryptase is also used as a molecular marker to study mast cell abundance, activation state, and effector functions in human tissues.
Mast Cell Tryptase CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TPSAB1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TPSAB1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TPSAB1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Mast Cell Tryptase protein expression.
This CRISPR knockout system enables efficient generation of TPSAB1-deficient cell models for investigation of Mast Cell Tryptase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TPSAB1 exon(s) critical for Mast Cell Tryptase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TPSAB1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Mast Cell Tryptase CRISPR/Cas9 KO Plasmid (h) and Mast Cell Tryptase CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TPSAB1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Mast Cell Tryptase HDR Plasmid (h) and Mast Cell Tryptase HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TPSAB1 homology arms to support homology-directed repair at defined TPSAB1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.