MAP-1B CRISPR/Cas9 KO Plasmid (h): sc-400981
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAP-1B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MAP-1B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAP-1B Antibody (H-8): sc-365668
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAP-1B CRISPR/Cas9 KO Plasmid (h) | sc-400981 | 20 µg | $397.00 |
Overview
MAP1B encodes microtubule-associated protein 1B (MAP-1B), a neuronal cytoskeletal regulator that binds microtubules and actin-associated complexes to support neurite outgrowth, axon guidance, and growth cone dynamics. MAP-1B contributes to microtubule stabilization, cytoskeletal remodeling, and intracellular transport processes that shape neuronal polarization and circuit formation. Its phosphorylation-dependent functions intersect with signaling pathways that govern cytoskeletal plasticity and synaptic development. Dysregulation of MAP1B expression or function has been linked to neurodevelopmental and neurodegenerative disease mechanisms, making it a useful target for studying neuronal structure–function relationships.
MAP-1B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MAP1B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MAP1B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MAP1B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MAP-1B protein expression.
This CRISPR knockout system enables efficient generation of MAP1B-deficient cell models for investigation of MAP-1B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MAP1B exon(s) critical for MAP-1B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MAP1B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MAP-1B CRISPR/Cas9 KO Plasmid (h) and MAP-1B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MAP1B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MAP-1B HDR Plasmid (h) and MAP-1B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MAP1B homology arms to support homology-directed repair at defined MAP1B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.