Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LZIP CRISPR/Cas9 KO Plasmid (h): sc-406526
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LZIP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LZIP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LZIP Antibody (Q-61): sc-100994
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LZIP CRISPR/Cas9 KO Plasmid (h) | sc-406526 | 20 µg | $397.00 |
Overview
CREB3 encodes LZIP, a basic leucine zipper transcription factor that localizes to the endoplasmic reticulum and can be activated by regulated intramembrane proteolysis to modulate gene expression. LZIP participates in cellular stress-adaptation programs, integrating ER stress and unfolded protein response signaling with transcriptional control of secretory pathway capacity and metabolic homeostasis. Through interactions with other bZIP factors and promoter elements, it influences pathways linked to inflammation, differentiation, and lipid and glucose metabolism. Dysregulated CREB3/LZIP activity has been associated in the literature with altered stress signaling and transcriptional reprogramming observed in multiple disease contexts, supporting its relevance for mechanistic studies.
LZIP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CREB3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CREB3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CREB3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LZIP protein expression.
This CRISPR knockout system enables efficient generation of CREB3-deficient cell models for investigation of LZIP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CREB3 exon(s) critical for LZIP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CREB3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LZIP CRISPR/Cas9 KO Plasmid (h) and LZIP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CREB3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LZIP HDR Plasmid (h) and LZIP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CREB3 homology arms to support homology-directed repair at defined CREB3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.