Ly-GDI/RhoGDI2/ARHGDIB Antibody (G-12): sc-376473

Ly-GDI Antibody (G-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ly-GDI in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Ly-GDI (G-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ly-GDI, also known as RhoGDI2 or ARHGDIB, plays a crucial role in regulating the activity of Rho family GTPases, which are essential for various cellular processes such as proliferation, cytoskeletal dynamics, and secretion. By binding to these GTPases, Ly-GDI inhibits their activation, thereby maintaining them in an inactive GDP-bound state. This regulation is vital for controlling cell signaling pathways, as dysregulation of Rho GTPases can lead to pathological conditions, including cancer and cardiovascular diseases. Notably, Ly-GDI is predominantly expressed in hematopoietic cells, particularly in B and T lymphocyte cell lines, highlighting its importance in immune responses and cellular signaling within the immune system.

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Ly-GDI/RhoGDI2/ARHGDIB Antibody (G-12) References:

1. Nuclear translocation of cleaved LyGDI dissociated from Rho and Rac during Trp53-dependent ionizing radiation-induced apoptosis of thymus cells in vitro.  |  Zhou, X., et al. 2004. Radiat Res. 162: 287-95. PMID: 15332996
2. Purification and N-terminal sequence of the p21rho GTPase-activating protein, rho GAP.  |  Garrett, MD., et al. 1991. Biochem J. 276 (Pt 3): 833-6. PMID: 1905930
3. The cellular functions of small GTP-binding proteins.  |  Hall, A. 1990. Science. 249: 635-40. PMID: 2116664
4. The GTPase superfamily: a conserved switch for diverse cell functions.  |  Bourne, HR., et al. 1990. Nature. 348: 125-32. PMID: 2122258
5. Ectopic expression of miR-34a enhances radiosensitivity of non-small cell lung cancer cells, partly by suppressing the LyGDI signaling pathway.  |  Duan, W., et al. 2013. J Radiat Res. 54: 611-9. PMID: 23349340
6. A cytoplasmic protein stimulates normal N-ras p21 GTPase, but does not affect oncogenic mutants.  |  Trahey, M. and McCormick, F. 1987. Science. 238: 542-5. PMID: 2821624
7. 2-O-Octadecylascorbic acid represses RhoGDIβ expression and ameliorates DNA damage-induced abnormal spindle orientations.  |  Doi, N., et al. 2021. J Cell Biochem. 122: 739-751. PMID: 33586155
8. A single residue can modify target-binding affinity and activity of the functional domain of the Rho-subfamily GDP dissociation inhibitors.  |  Platko, JV., et al. 1995. Proc Natl Acad Sci U S A. 92: 2974-8. PMID: 7708758
9. Ly-GDI, a GDP-dissociation inhibitor of the RhoA GTP-binding protein, is expressed preferentially in lymphocytes.  |  Scherle, P., et al. 1993. Proc Natl Acad Sci U S A. 90: 7568-72. PMID: 8356058
10. Identification of a GDI displacement factor that releases endosomal Rab GTPases from Rab-GDI.  |  Dirac-Svejstrup, AB., et al. 1997. EMBO J. 16: 465-72. PMID: 9034329
11. RhoGDIgamma: a GDP-dissociation inhibitor for Rho proteins with preferential expression in brain and pancreas.  |  Adra, CN., et al. 1997. Proc Natl Acad Sci U S A. 94: 4279-84. PMID: 9113980

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ly-GDI/RhoGDI2/ARHGDIB Antibody (G-12) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Ly-GDI/RhoGDI2/ARHGDIB Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ly-GDI/RhoGDI2/ARHGDIB Antibody (G-12) Specifications

Alternate Product Name: Ly-GDI

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 159-189

Epitope Target: Ly-GDI

Epitope Species: human

Product Reactivity/Detection: Ly-GDI

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Ly-GDI/RhoGDI2/ARHGDIB Antibody (G-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin