Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LRRC8D CRISPR/Cas9 KO Plasmid (h): sc-406426
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LRRC8D CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LRRC8D genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LRRC8D Antibody (A-12): sc-515070
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LRRC8D CRISPR/Cas9 KO Plasmid (h) | sc-406426 | 20 µg | $397.00 |
Overview
LRRC8D encodes a leucine-rich repeat–containing subunit of the volume-regulated anion channel (VRAC), a heteromeric complex that mediates swelling-activated anion flux and contributes to cell volume homeostasis. LRRC8D-containing VRAC assemblies influence transport of osmolytes and small anions and can modulate redox balance and cellular stress responses, intersecting with processes such as proliferation, apoptosis, and inflammatory signaling. In immune and barrier tissues, VRAC composition including LRRC8D has been linked to regulation of reactive oxygen species handling and stimulus-dependent signaling outputs. Dysregulated VRAC activity and altered LRRC8 subunit expression patterns have been investigated in contexts including cancer biology, metabolic stress, and immune-mediated pathophysiology, where ion transport and oxidative stress pathways are frequently remodeled.
LRRC8D CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LRRC8D gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LRRC8D together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LRRC8D open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LRRC8D protein expression.
This CRISPR knockout system enables efficient generation of LRRC8D-deficient cell models for investigation of LRRC8D signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LRRC8D exon(s) critical for LRRC8D function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LRRC8D genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LRRC8D CRISPR/Cas9 KO Plasmid (h) and LRRC8D CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LRRC8D locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LRRC8D HDR Plasmid (h) and LRRC8D HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LRRC8D homology arms to support homology-directed repair at defined LRRC8D target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.