LAMP-2 Antibody (6A430): sc-71492

LAMP-2 Antibody (6A430) is a rat monoclonal IgG1 antibody targeting an epitope mapped to an immunoadsorbent-purified mouse macrophage glycoprotein fraction, with reactivity in mouse, rat, and human species. LAMP-2 monoclonal antibody (6A430) is validated for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). LAMP-2, also known as CD107B, is a glycosylated type I transmembrane protein found primarily on the lysosomal membrane, where LAMP-2 plays a crucial role in lysosomal integrity and autophagy. LAMP-2 facilitates the fusion of autophagosomes with lysosomes and interacts with key proteins involved in intracellular trafficking, such as Rab7, which regulates late endosome to lysosome transport. LAMP-2 also associates with lysosomal enzymes like cathepsins, supporting their stability and activity within the acidic lysosomal environment. Additionally, LAMP-2′s interaction with mannose-6-phosphate receptors highlights LAMP-2′s role in the sorting and delivery of hydrolases to lysosomes. Dysregulation of LAMP-2 expression or LAMP-2′s interactions can lead to lysosomal storage disorders such as Danon disease and is implicated in tumor progression due to LAMP-2′s role in cellular adhesion and invasion. Anti-LAMP-2 antibody (6A430) is a valuable resource for studying LAMP-2′s functional interactions and significance in lysosomal biology and disease.

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LAMP-2 Antibody (6A430) References:

1. Asparagine-linked oligosaccharides protect Lamp-1 and Lamp-2 from intracellular proteolysis.  |  Kundra, R. and Kornfeld, S. 1999. J Biol Chem. 274: 31039-46. PMID: 10521503
2. Influences of the lysosomal associated membrane proteins (Lamp-1, Lamp-2) and Mac-2 binding protein (Mac-2-BP) on the prognosis of pancreatic carcinoma.  |  Künzli, BM., et al. 2002. Cancer. 94: 228-39. PMID: 11815981
3. Genetic analysis of the LAMP-2 gene promoter in patients with sporadic Parkinson's disease.  |  Pang, S., et al. 2012. Neurosci Lett. 526: 63-7. PMID: 22867958
4. Two human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2, are encoded by genes localized to chromosome 13q34 and chromosome Xq24-25, respectively.  |  Mattei, MG., et al. 1990. J Biol Chem. 265: 7548-51. PMID: 2332441
5. The ceramide analogue N-(1-hydroxy-3-morpholino-1-phenylpropan-2-yl)decanamide induces large lipid droplet accumulation and highlights the effect of LAMP-2 deficiency on lipid droplet degradation.  |  Kato, Y., et al. 2020. Bioorg Med Chem Lett. 30: 126891. PMID: 31874824
6. Danon Disease Presenting with Slowly Progressive Cardiomyopathy and Harboring a Novel Missense Variant in the Lysosome-associated Membrane Protein Type 2 (LAMP-2) gene: A Case Report.  |  Nakagawa, Y., et al. 2024. Intern Med.. PMID: 39111889
7. The lysosomal membrane glycoproteins Lamp-1 and Lamp-2 are present in mobilizable organelles, but are absent from the azurophil granules of human neutrophils.  |  Dahlgren, C., et al. 1995. Biochem J. 311 (Pt 2): 667-74. PMID: 7487911
8. The family of LAMP-2 proteins arises by alternative splicing from a single gene: characterization of the avian LAMP-2 gene and identification of mammalian homologs of LAMP-2b and LAMP-2c.  |  Gough, NR., et al. 1995. DNA Cell Biol. 14: 863-7. PMID: 7546292
9. Increased LAMP-2 polylactosamine glycosylation is associated with its slower Golgi transit during establishment of a polarized MDCK epithelial monolayer.  |  Nabi, IR. and Rodriguez-Boulan, E. 1993. Mol Biol Cell. 4: 627-35. PMID: 8374171
10. Biosynthetic transport of a major lysosome-associated membrane glycoprotein 2, lamp-2: a significant fraction of newly synthesized lamp-2 is delivered to lysosomes by way of early endosomes.  |  Akasaki, K., et al. 1996. J Biochem. 120: 1088-94. PMID: 9010755

The preferred LAMP-2 antibody is LAMP-2 Antibody (H4B4): sc-18822.