Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Laminin β-3 Double Nickase Plasmid (h): sc-402636-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Laminin β-3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Laminin β-3 Double Nickase Plasmid (h) and Laminin β-3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LAMB3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Laminin β-3 Antibody (A-6): sc-133178
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Laminin β-3 Double Nickase Plasmid (h) | sc-402636-NIC | 20 µg | $410.00 | |||
Laminin β-3 Double Nickase Plasmid (h2) | sc-402636-NIC-2 | 20 µg | $410.00 |
LAMB3 encodes laminin β-3, an essential subunit of laminin-332 (α3β3γ2), a basement membrane glycoprotein that organizes extracellular matrix architecture and promotes epithelial adhesion, polarity, and migration. Through interactions with integrins such as α3β1 and α6β4, laminin-332 supports hemidesmosome assembly and modulates focal adhesion signaling pathways that influence cytoskeletal dynamics and tissue integrity. LAMB3-dependent matrix cues are central to epidermal and mucosal barrier function, and altered laminin-332 expression or organization has been associated with skin fragility disorders and invasive behavior in epithelial tumors. These features make LAMB3 a widely used target for studying basement membrane biology, cell–matrix signaling, and epithelial remodeling.
Laminin β-3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LAMB3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LAMB3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LAMB3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LAMB3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.