Lamin A Double Nickase Plasmid (h): sc-400039-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Lamin A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Lamin A Double Nickase Plasmid (h) and Lamin A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LMNA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Lamin A Antibody (C-3): sc-518013
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lamin A Double Nickase Plasmid (h) | sc-400039-NIC | 20 µg | $410.00 | |||
Lamin A Double Nickase Plasmid (h2) | sc-400039-NIC-2 | 20 µg | $410.00 |
Human LMNA encodes lamin A, a core component of the nuclear lamina that provides mechanical stability and organizes higher-order chromatin architecture at the nuclear periphery. Lamin A participates in genome regulation through lamin-associated domains (LADs), influencing transcriptional programs, DNA replication timing, and epigenetic state, and it supports nuclear envelope integrity during the cell cycle. LMNA interfaces with DNA damage signaling and mechanotransduction pathways, linking cytoskeletal forces to nuclear shape and gene expression. Disruption of lamin A homeostasis is associated with diverse laminopathies, including cardiomyopathy and muscular dystrophy spectra, premature aging phenotypes, and altered nuclear morphology relevant to cancer and stem cell biology.
Lamin A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LMNA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LMNA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LMNA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LMNA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.