Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
L-type Ca++ CP γ8 CRISPR/Cas9 KO Plasmid (h): sc-407391
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- L-type Ca++ CP γ8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the L-type Ca++ CP γ8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: L-type Ca++ CP γ8 Antibody (A-8): sc-514421
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
L-type Ca++ CP γ8 CRISPR/Cas9 KO Plasmid (h) | sc-407391 | 20 µg | $397.00 |
Overview
CACNG8 encodes the L-type Ca++ channel auxiliary subunit γ8, a transmembrane regulator that modulates trafficking, gating, and membrane stability of voltage-gated calcium channel complexes in excitable cells. By tuning Ca2+ influx, γ8 influences calcium-dependent signaling networks that couple membrane depolarization to downstream pathways controlling neuronal excitability, synaptic transmission, and activity-dependent gene expression. Altered CACNG8 function has been implicated in neurophysiological phenotypes and may contribute to mechanisms relevant to seizure susceptibility and other disorders linked to dysregulated calcium signaling. As a component of ion channel macromolecular assemblies, CACNG8 provides a handle for studying channel complex composition and the cellular consequences of perturbing auxiliary subunits.
L-type Ca++ CP γ8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CACNG8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CACNG8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CACNG8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish L-type Ca++ CP γ8 protein expression.
This CRISPR knockout system enables efficient generation of CACNG8-deficient cell models for investigation of L-type Ca++ CP γ8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CACNG8 exon(s) critical for L-type Ca++ CP γ8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CACNG8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by L-type Ca++ CP γ8 CRISPR/Cas9 KO Plasmid (h) and L-type Ca++ CP γ8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CACNG8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by L-type Ca++ CP γ8 HDR Plasmid (h) and L-type Ca++ CP γ8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CACNG8 homology arms to support homology-directed repair at defined CACNG8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.