Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
KV2.2 CRISPR/Cas9 KO Plasmid (h): sc-409737
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KV2.2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KV2.2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KV2.2 Antibody (E-7): sc-376275
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KV2.2 CRISPR/Cas9 KO Plasmid (h) | sc-409737 | 20 µg | $397.00 |
Overview
KCNB2 encodes the voltage-gated potassium channel subunit KV2.2, a delayed rectifier channel that shapes membrane repolarization and regulates neuronal firing patterns. KV2.2 contributes to control of action potential frequency, afterhyperpolarization, and excitability-dependent signaling, linking electrical activity to calcium dynamics and downstream transcriptional responses. In the central nervous system, KV2-family channels influence synaptic integration and network excitability, and altered potassium channel function is broadly associated with neurological dysfunction and susceptibility to seizure and neurodevelopmental phenotypes. Human KCNB2 is therefore a useful target for dissecting ion channel–dependent mechanisms that couple membrane potential to neuronal physiology.
KV2.2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KCNB2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KCNB2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KCNB2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KV2.2 protein expression.
This CRISPR knockout system enables efficient generation of KCNB2-deficient cell models for investigation of KV2.2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KCNB2 exon(s) critical for KV2.2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KCNB2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KV2.2 CRISPR/Cas9 KO Plasmid (h) and KV2.2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KCNB2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KV2.2 HDR Plasmid (h) and KV2.2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KCNB2 homology arms to support homology-directed repair at defined KCNB2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.