JMJD5 CRISPR/Cas9 KO Plasmid (h): sc-405787
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- JMJD5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the JMJD5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: JMJD5 Antibody (D-5): sc-377078
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JMJD5 CRISPR/Cas9 KO Plasmid (h) | sc-405787 | 20 µg | $397.00 |
Overview
KDM8 encodes the Jumonji C domain–containing protein JMJD5, a nuclear oxygenase implicated in chromatin regulation and transcriptional control through histone lysine hydroxylation/demethylation-associated mechanisms. JMJD5 has been linked to cell-cycle progression, replication stress responses, and coordination of gene expression programs that intersect with DNA damage signaling and metabolic adaptation. Through these functions, JMJD5 can influence proliferation-associated pathways and epigenetic states that are frequently altered in cancer and other disorders involving dysregulated chromatin dynamics. As a result, KDM8/JMJD5 is widely studied as a node connecting epigenetic regulation to context-dependent transcriptional outputs and cellular fitness.
JMJD5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KDM8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KDM8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KDM8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish JMJD5 protein expression.
This CRISPR knockout system enables efficient generation of KDM8-deficient cell models for investigation of JMJD5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KDM8 exon(s) critical for JMJD5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KDM8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by JMJD5 CRISPR/Cas9 KO Plasmid (h) and JMJD5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KDM8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by JMJD5 HDR Plasmid (h) and JMJD5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KDM8 homology arms to support homology-directed repair at defined KDM8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.