JAK2 Double Nickase Plasmid (h): sc-400246-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- JAK2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- JAK2 Double Nickase Plasmid (h) and JAK2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting JAK2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: JAK2 Antibody (C-10): sc-390539
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JAK2 Double Nickase Plasmid (h) | sc-400246-NIC | 20 µg | $410.00 | |||
JAK2 Double Nickase Plasmid (h2) | sc-400246-NIC-2 | 20 µg | $410.00 |
Human JAK2 encodes a non-receptor tyrosine kinase that couples cytokine and growth factor receptors to intracellular signaling, most prominently through the JAK–STAT pathway. Upon receptor engagement, JAK2 mediates phosphorylation cascades that influence transcriptional programs governing hematopoiesis, immune cell differentiation, and inflammatory responses, with additional crosstalk into PI3K–AKT and MAPK signaling. Dysregulated JAK2 activity or expression has been linked to altered cytokine sensitivity and aberrant proliferative signaling in hematologic and inflammatory disease biology. As a central node in receptor-proximal signaling, JAK2 is widely studied for its role in lineage commitment, stress signaling, and transcriptional control downstream of cytokine receptors.
JAK2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the JAK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within JAK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt JAK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of JAK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.