Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IRS-2 CRISPR/Cas9 KO Plasmid (h): sc-400474
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IRS-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IRS-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IRS-2 Antibody (B-5): sc-390761
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IRS-2 CRISPR/Cas9 KO Plasmid (h) | sc-400474 | 20 µg | $397.00 |
Overview
Insulin receptor substrate 2 (IRS-2) is a cytoplasmic adaptor protein that couples activated insulin and IGF-1 receptors to downstream signaling through PI3K–AKT and RAS–MAPK pathways. By undergoing receptor-mediated tyrosine phosphorylation, IRS2 recruits SH2-domain effectors to regulate glucose metabolism, lipid homeostasis, cell growth, survival, and differentiation. IRS2-dependent signaling contributes to hepatic insulin action, pancreatic β-cell function, and energy balance, and dysregulation is frequently studied in insulin resistance, type 2 diabetes, obesity, and related metabolic phenotypes. Altered IRS2 signaling has also been implicated in context-dependent effects on proliferation and motility, supporting its use in pathway and disease-mechanism research.
IRS-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IRS2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IRS2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IRS2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IRS-2 protein expression.
This CRISPR knockout system enables efficient generation of IRS2-deficient cell models for investigation of IRS-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IRS2 exon(s) critical for IRS-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IRS2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IRS-2 CRISPR/Cas9 KO Plasmid (h) and IRS-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IRS2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IRS-2 HDR Plasmid (h) and IRS-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IRS2 homology arms to support homology-directed repair at defined IRS2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.