Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin αL/ITGAL/CD11a Double Nickase Plasmid (h): sc-402246-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin αL/ITGAL/CD11a Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin αL/ITGAL/CD11a Double Nickase Plasmid (h) and Integrin αL/ITGAL/CD11a Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGAL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin αL/ITGAL/CD11a Antibody (E-1): sc-374172
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin αL/ITGAL/CD11a Double Nickase Plasmid (h) | sc-402246-NIC | 20 µg | $410.00 | |||
Integrin αL/ITGAL/CD11a Double Nickase Plasmid (h2) | sc-402246-NIC-2 | 20 µg | $410.00 |
ITGAL encodes integrin αL (CD11a), which heterodimerizes with integrin β2 (CD18) to form LFA-1, a leukocyte adhesion receptor essential for immune cell trafficking and cell–cell interactions. LFA-1 binding to ICAM family ligands regulates firm adhesion, transendothelial migration, and formation of the immunological synapse, coupling extracellular cues to cytoskeletal remodeling and signaling pathways such as outside-in integrin signaling, SRC family kinase activation, and focal adhesion-associated processes. ITGAL activity influences lymphocyte activation thresholds, antigen-dependent effector functions, and coordination of inflammatory responses. Dysregulated LFA-1–ICAM interactions and altered ITGAL expression are implicated in immune-mediated pathology and leukocyte adhesion defects, supporting its use in studies of inflammation, autoimmunity, and tumor–immune microenvironment dynamics.
Integrin αL/ITGAL/CD11a Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGAL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGAL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGAL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGAL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.