Integrin β4/ITGB4/CD104 Double Nickase Plasmid (h): sc-400573-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin β4/ITGB4/CD104 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin β4/ITGB4/CD104 Double Nickase Plasmid (h) and Integrin β4/ITGB4/CD104 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGB4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin β4/ITGB4/CD104 Antibody (B-7): sc-514426
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin β4/ITGB4/CD104 Double Nickase Plasmid (h) | sc-400573-NIC | 20 µg | $410.00 | |||
Integrin β4/ITGB4/CD104 Double Nickase Plasmid (h2) | sc-400573-NIC-2 | 20 µg | $410.00 |
ITGB4 encodes integrin β4 (CD104), a transmembrane adhesion receptor that pairs predominantly with integrin α6 to form α6β4, a core component of hemidesmosomes linking epithelial cells to the basement membrane via laminins. Through coupling to intermediate filaments and crosstalk with focal adhesion and growth factor signaling, ITGB4 regulates cell polarity, cytoskeletal organization, and migration, with downstream effects on PI3K–AKT, MAPK, and Rho GTPase–dependent pathways. Altered ITGB4 expression or localization is associated with dysregulated epithelial integrity and increased invasive behavior in multiple carcinoma contexts, and pathogenic variants are linked to blistering disorders involving compromised dermal–epidermal adhesion. These biology features make ITGB4 a useful target for studying adhesion-dependent signaling, epithelial remodeling, and extracellular matrix–driven phenotypes.
Integrin β4/ITGB4/CD104 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGB4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGB4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGB4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGB4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.