Integrin α4/ITGA4/CD49d CRISPR/Cas9 KO Plasmid (m): sc-421162

Overview

Itga4 encodes integrin α4 (ITGA4/CD49d), an α subunit that pairs with β1 or β7 to form the α4β1 (VLA-4) and α4β7 integrins, central mediators of cell–cell and cell–matrix adhesion. These receptors bind ligands such as VCAM1 and fibronectin, coordinating leukocyte trafficking, firm adhesion, and transendothelial migration during immune surveillance and inflammation. ITGA4 signaling integrates with focal adhesion and cytoskeletal remodeling pathways to influence migration, survival, and activation programs, and it contributes to hematopoietic and lymphoid cell positioning within tissues. Dysregulated α4 integrin activity is widely studied in inflammatory and autoimmune pathobiology, as well as in tumor–stromal interactions that support invasion and metastatic dissemination.

Integrin α4/ITGA4/CD49d CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Itga4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Itga4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Itga4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Integrin α4/ITGA4/CD49d protein expression.

This CRISPR knockout system enables efficient generation of Itga4-deficient cell models for investigation of Integrin α4/ITGA4/CD49d signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Itga4 exon(s) critical for Integrin α4/ITGA4/CD49d function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Itga4 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Integrin α4/ITGA4/CD49d CRISPR/Cas9 KO Plasmid (m) and Integrin α4/ITGA4/CD49d CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Itga4 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Integrin α4/ITGA4/CD49d HDR Plasmid (m) and Integrin α4/ITGA4/CD49d HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Itga4 homology arms to support homology-directed repair at defined Itga4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.