Integrin β3/ITGB3/CD61 Double Nickase Plasmid (h): sc-400216-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin β3/ITGB3/CD61 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin β3/ITGB3/CD61 Double Nickase Plasmid (h) and Integrin β3/ITGB3/CD61 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGB3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin β3/ITGB3/CD61 Antibody (D-11): sc-365679
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin β3/ITGB3/CD61 Double Nickase Plasmid (h) | sc-400216-NIC | 20 µg | $410.00 | |||
Integrin β3/ITGB3/CD61 Double Nickase Plasmid (h2) | sc-400216-NIC-2 | 20 µg | $410.00 |
ITGB3 encodes integrin β3 (CD61), a transmembrane adhesion receptor that heterodimerizes with integrin αIIb or αV to form αIIbβ3 and αVβ3 complexes that bind extracellular matrix ligands and transmit bidirectional signaling across the plasma membrane. Through coupling to focal adhesion and cytoskeletal regulators, integrin β3 modulates cell adhesion, spreading, migration, and mechanotransduction, engaging pathways such as FAK/SRC, PI3K–AKT, and Rho family GTPases. In platelets and megakaryocytes, αIIbβ3 signaling is central to aggregation and thrombus formation, while αVβ3 contributes to endothelial and tumor cell interactions with the matrix. Altered ITGB3 expression or function has been linked to dysregulated hemostasis, vascular biology, inflammation, and cancer-associated invasion and metastasis, making it a widely studied node in adhesion-dependent signaling networks.
Integrin β3/ITGB3/CD61 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGB3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGB3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGB3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGB3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.