Integrin α3/ITGA3/CD49c CRISPR/Cas9 KO Plasmid (m2): sc-421161-KO-2

Overview

Itga3 encodes integrin α3 (ITGA3/CD49c), an α subunit that pairs with β1 to form the laminin-binding α3β1 integrin, a key mediator of cell–extracellular matrix adhesion. Through focal adhesion signaling, ITGA3 influences cytoskeletal organization and activates downstream pathways such as FAK/Src, PI3K–AKT, and MAPK to regulate migration, survival, and epithelial morphogenesis. In mouse tissues, integrin α3 contributes to basement membrane interactions and epithelial barrier architecture, linking it to processes such as wound-associated remodeling and cell polarity. Altered ITGA3-integrin signaling is frequently studied in contexts of invasive behavior, inflammation-associated tissue remodeling, and organ developmental phenotypes.

Integrin α3/ITGA3/CD49c CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Itga3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Itga3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Itga3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Integrin α3/ITGA3/CD49c protein expression.

This CRISPR knockout system enables efficient generation of Itga3-deficient cell models for investigation of Integrin α3/ITGA3/CD49c signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Itga3 exon(s) critical for Integrin α3/ITGA3/CD49c function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Itga3 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Integrin α3/ITGA3/CD49c CRISPR/Cas9 KO Plasmid (m) and Integrin α3/ITGA3/CD49c CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Itga3 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Integrin α3/ITGA3/CD49c HDR Plasmid (m) and Integrin α3/ITGA3/CD49c HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Itga3 homology arms to support homology-directed repair at defined Itga3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.