Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ING1 Double Nickase Plasmid (h): sc-402893-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ING1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ING1 Double Nickase Plasmid (h) and ING1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ING1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ING1 Antibody (E-10): sc-373817
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ING1 Double Nickase Plasmid (h) | sc-402893-NIC | 20 µg | $410.00 | |||
ING1 Double Nickase Plasmid (h2) | sc-402893-NIC-2 | 20 µg | $410.00 |
ING1 (inhibitor of growth family member 1) encodes a nuclear tumor suppressor–associated protein that functions as a chromatin reader through its PHD finger, recognizing H3K4me3 and coordinating transcriptional and epigenetic control. ING1 integrates signals from DNA damage and cellular stress pathways, linking p53-dependent responses with regulation of cell-cycle progression, apoptosis, and senescence. It participates in chromatin remodeling and histone acetylation/deacetylation complexes, helping shape gene expression programs that maintain genome stability. Altered ING1 expression or function has been reported across multiple malignancies and is studied in the context of impaired DNA repair capacity and dysregulated growth control.
ING1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ING1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ING1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ING1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ING1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.