Date published: 2025-10-5
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western blotting image from recommended NFKBIA/IkB alpha (H-4) monoclonal antibody replacement. Image 354367
Este anticorpo policlonal foi descontinuado. Consulta o nosso anticorpo monoclonal NFKBIA/IkB alpha (H-4) recomendado (dados de amostra apresentados).
western blotting image from recommended NFKBIA/IkB alpha (H-4) monoclonal antibody replacement. Image 354367
Este anticorpo policlonal foi descontinuado. Consulta o nosso anticorpo monoclonal NFKBIA/IkB alpha (H-4) recomendado (dados de amostra apresentados).
Este anticorpo policlonal foi descontinuado. Consulta o nosso anticorpo monoclonal NFKBIA/IkB alpha (H-4) recomendado (dados de amostra apresentados).

IκB-α Anticorpo (C-21): sc-371

4.8(30)
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  • I kappa B- alpha Anticorpo C-21 é um anticorpo policlonal produzido em coelho IgG fornecido em100 µg/ml; Também disponível produzido em cabra IgG fornecido em 200 µg/ml, sc-371-G
  • epítopo mapeado no C-terminus de IκB-α de human origem
  • Anticorpo policlonal descontinuado

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Descricao

VEJA TAMBÉM

Adicionais I κ B incluindo Anticorpos I kappa B-α, I kappa B-β e I κ B-ε

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

Informacoes sobre ordens

NFKBIA/IkB alpha (H-4): sc-1643 [ anticorpo monoclonal substituto recomendado para IκB-α (C-21) ]

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

NFKBIA/IkB alpha Anticorpo (H-4)

sc-1643
200 µg/ml
$316.00

Pacote do NFKBIA/IkB alpha (H-4): m-IgG Fc BP-HRP

sc-528129
200 µg Ab; 10 µg BP
$354.00

Pacote do NFKBIA/IkB alpha (H-4): m-IgGκ BP-HRP

sc-520432
200 µg Ab, 40 µg BP
$354.00

NFKBIA/IkB alpha Anticorpo (H-4) AC

sc-1643 AC
500 µg/ml, 25% agarose
$416.00

NFKBIA/IkB alpha Anticorpo (H-4) HRP

sc-1643 HRP
200 µg/ml
$316.00

NFKBIA/IkB alpha Anticorpo (H-4) FITC

sc-1643 FITC
200 µg/ml
$330.00

NFKBIA/IkB alpha Anticorpo (H-4) PE

sc-1643 PE
200 µg/ml
$343.00

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor® 488

sc-1643 AF488
200 µg/ml
$357.00

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor® 546

sc-1643 AF546
200 µg/ml
$357.00

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor® 594

sc-1643 AF594
200 µg/ml
$357.00

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor® 647

sc-1643 AF647
200 µg/ml
$357.00

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor® 680

sc-1643 AF680
200 µg/ml
$357.00

NFKBIA/IkB alpha Anticorpo (H-4) Alexa Fluor® 790

sc-1643 AF790
200 µg/ml
$357.00

NFKBIA/IkB alpha Anticorpo (H-4) TRITC

sc-1643 TRITC
200 µg/ml
$320.00

Hi, I've tried this antibody in WB using 30 ug of rat  liver cytoplasm lysate. I blocked nitrocellulose membrane with 10% skim milk for 1 h followed by overnight incubation with 1:100 primary antibody. Unfortunately I saw only a band at 90kDa.

Asked by: dany58
Thanks for your feedback. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge. Please contact our Technical Service by phone or email or by Live Chat if you need support or additional information
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

hi, i keep getting two bands using this antibody. one band appears at around 37 kda and another one above 37 kda. could you explain what i can do to solve this problem? i'm using hacat (human keratinocyte cell line), 5% skim milk for blocking thank you

Asked by: shiba
Thank you for your question about our rabbit polyclonal antibody, sc-371: IkB-alpha (C-21). The expected molecular weight for IkB-alpha is approximately 37 kDa. This protein may also be phosphorylated which is one possible explanation for why you may also be detecting an additional slightly higher molecular weight band in your samples. I would recommend including additional controls in your experiment to confirm if the additional band above 37 kDa is also IkB-alpha, and a result of the binding of the specific primary antibody. One suggestion is to treat your lysates with Lambda Phosphatase: sc-200312 (https://www.scbt.com/scbt/product/lambda-phosphatase?requestFrom=search) to release phosphate groups and observe the difference in detection by Western blot. I would also recommend to perform a secondary antibody control blot (without primary antibody) to confirm that there is not any non-specific binding of your secondary antibody alone. Please contact our Technical Service Department for additional support in troubleshooting this antibody in your research application. https://www.scbt.com/scbt/resources/technical-service
Answered by: Technical Support
Date published: 2018-12-18
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Rated 5 out of 5 by from Good for western blotFor western blot, use at 1:1000 in 5% milk TBST, overnight incubation with rocking at 4 degrees C. Example of published result: PMID 29950426
Date published: 2020-01-09
Rated 5 out of 5 by from IkB antibody in rat samplesIt is working very well in the rat samples without unspecific bands
Date published: 2019-08-02
Rated 5 out of 5 by from quality of anti-IkBa antibodyThis antibody's quality is very good, and works very well in our hand.
Date published: 2019-01-15
Rated 4 out of 5 by from Nice primary antibodyThe primary antibody is available for Western blotting in mice sample.
Date published: 2017-06-13
Rated 5 out of 5 by from Works really wellI have used this antibody for nearly five years and it worked really well with bone-marrow derived dendritic cell extracts of BALB/c and C57BL/6 mice. There were no non-specific bands and the band intensity for the specific bands was also very good.
Date published: 2017-06-06
Rated 4 out of 5 by from Excellent experience.I bought this antobody for my current research project involved in the IkB-alpha protein detection by western blot assay for the mice kidney samples, this antibody works very well!
Date published: 2017-02-12
Rated 5 out of 5 by from Very clean immunoblots and high sensitivityThis is a great antibody for immunoblot analisis of endogenous IkBalpha. Very clean western blots and it can be diluted even 1:5000.
Date published: 2016-12-21
Rated 5 out of 5 by from Great result in WBGreat result in WB using human and rat lungs tissue.
Date published: 2016-11-04
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IκB-α Anticorpo (C-21) is rated 4.8 out of 5 by 30.
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