IFIT1 Double Nickase Plasmid (m): sc-421035-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IFIT1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IFIT1 Double Nickase Plasmid (m) and IFIT1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ifit1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFIT1 Double Nickase Plasmid (m) | sc-421035-NIC | 20 µg | $410.00 | |||
IFIT1 Double Nickase Plasmid (m2) | sc-421035-NIC-2 | 20 µg | $410.00 |
Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), encoded by mouse Ifit1, is a key interferon-stimulated effector that restricts viral replication by recognizing non-self RNA features such as improperly capped or 5′-triphosphate RNA and limiting translation initiation. IFIT1 functions within innate immune signaling downstream of type I interferon and pattern-recognition receptor pathways, including RIG-I–like receptor and TLR-driven antiviral responses, shaping the transcriptional and translational landscape during infection and inflammation. Altered Ifit1 expression and IFIT family activity have been linked to dysregulated interferon signatures, host susceptibility to viral pathogens, and immune-mediated pathology, making it a useful node for dissecting interferon-dependent gene programs in mouse models. Researchers commonly interrogate IFIT1 in contexts such as cytokine-driven stress responses, macrophage and dendritic cell activation, and modulation of antigen presentation and immune cell cross-talk.
IFIT1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ifit1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ifit1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ifit1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ifit1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.