IFIT1 Double Nickase Plasmid (h): sc-403066-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IFIT1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IFIT1 Double Nickase Plasmid (h) and IFIT1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IFIT1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFIT1 Double Nickase Plasmid (h) | sc-403066-NIC | 20 µg | $410.00 | |||
IFIT1 Double Nickase Plasmid (h2) | sc-403066-NIC-2 | 20 µg | $410.00 |
Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) is an interferon-stimulated gene product that binds capped and incompletely 2′-O-methylated viral and cellular RNAs to restrict translation and support innate antiviral defense. IFIT1 functions within type I/III interferon signaling downstream of JAK–STAT activation, cooperating with other IFIT family members to discriminate self versus non-self RNA and modulate RNA metabolism and ribosome engagement. Through these activities, IFIT1 contributes to host–pathogen interactions, regulation of inflammatory programs, and shaping of cellular responses to nucleic acid sensing pathways. Altered IFIT1 expression and interferon pathway dysregulation are frequently observed in contexts of chronic inflammation, autoimmune interferon signatures, and infection-associated immune remodeling, making IFIT1 a useful node for mechanistic studies.
IFIT1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IFIT1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IFIT1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IFIT1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IFIT1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.