I1PP2A Double Nickase Plasmid (h): sc-402988-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANP32A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ANP32A Double Nickase Plasmid (h) and ANP32A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ANP32A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ANP32A Antibody (A-182): sc-100767
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
I1PP2A Double Nickase Plasmid (h) | sc-402988-NIC | 20 µg | $410.00 | |||
ANP32A Double Nickase Plasmid (h2) | sc-402988-NIC-2 | 20 µg | $410.00 |
ANP32A encodes I1PP2A, an acidic leucine-rich nuclear phosphoprotein that functions as an inhibitor of protein phosphatase 2A (PP2A) and a regulator of chromatin-associated processes. By modulating PP2A-dependent dephosphorylation events, I1PP2A influences cell cycle progression, DNA damage responses, and signaling nodes that integrate transcriptional control with cellular stress adaptation. ANP32A also participates in mRNA metabolism and nucleocytoplasmic dynamics through interactions with RNA-binding and chromatin remodeling factors. Dysregulated ANP32A/I1PP2A activity has been linked in the literature to altered proliferation and survival phenotypes, supporting its relevance to studies of oncogenic signaling and neurodegenerative pathways.
ANP32A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ANP32A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ANP32A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ANP32A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ANP32A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.