Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HuR/ELAV1 Double Nickase Plasmid (h): sc-400141-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HuR/ELAV1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HuR/ELAV1 Double Nickase Plasmid (h) and HuR/ELAV1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ELAVL1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HuR/ELAV1 Double Nickase Plasmid (h) | sc-400141-NIC | 20 µg | $410.00 | |||
HuR/ELAV1 Double Nickase Plasmid (h2) | sc-400141-NIC-2 | 20 µg | $410.00 |
ELAVL1 (HuR/ELAV1) encodes an AU-rich element–binding RNA-binding protein that regulates post-transcriptional gene expression by controlling mRNA stability, splicing, nuclear export, and translation. HuR shuttles between nucleus and cytoplasm and is modulated by stress-responsive signaling and phosphorylation, shaping gene programs involved in cell cycle progression, DNA damage responses, inflammation, and differentiation. By stabilizing transcripts encoding cytokines, growth factors, and survival regulators, HuR integrates RNA regulons that influence cellular adaptation to microenvironmental cues. Dysregulated ELAVL1 activity and altered HuR localization have been associated with oncogenic signaling, chronic inflammatory states, and neurodegenerative and cardiovascular disease–relevant pathways, supporting its broad use as a mechanistic node in RNA biology.
HuR/ELAV1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ELAVL1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ELAVL1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ELAVL1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ELAVL1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.