Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Hec1 CRISPR/Cas9 KO Plasmid (h): sc-406844
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hec1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Hec1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Hec1 Antibody (C-11): sc-515550
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hec1 CRISPR/Cas9 KO Plasmid (h) | sc-406844 | 20 µg | $397.00 |
Overview
NDC80 encodes Hec1, an essential component of the outer kinetochore that forms the NDC80 complex and directly couples spindle microtubules to chromosomes during mitosis. Hec1 coordinates chromosome congression and segregation by supporting stable end-on microtubule attachments and contributing to spindle assembly checkpoint signaling, thereby maintaining genome integrity. Disruption or dysregulation of Hec1 perturbs mitotic progression, increases chromosomal instability, and intersects with pathways controlling aneuploidy and cell-cycle checkpoints. These processes are frequently examined in studies of proliferative disorders and tumor cell biology where kinetochore function and mitotic fidelity are altered.
Hec1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NDC80 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NDC80 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NDC80 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Hec1 protein expression.
This CRISPR knockout system enables efficient generation of NDC80-deficient cell models for investigation of Hec1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NDC80 exon(s) critical for Hec1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NDC80 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Hec1 CRISPR/Cas9 KO Plasmid (h) and Hec1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NDC80 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Hec1 HDR Plasmid (h) and Hec1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NDC80 homology arms to support homology-directed repair at defined NDC80 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.