GW182 Antibody (E-1): sc-376939
- GW182 Antibody (E-1) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1856-1925 mapping near the C-terminus of GW182 of human origin
- recommended for detection of GW182 isoforms 1-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
- See GW182 (A-6): sc-374458 for GW182 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GW182 Antibody (E-1) for WB and IHC(P) applications. These reagents are now offered in bundles with GW182 Antibody (E-1) (see ordering information below).
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GW182 Antibody (E-1) is a mouse monoclonal IgG1 antibody that detects GW182 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GW182, also known as the EMSY interactor protein, plays a pivotal role in the formation and maintenance of GW bodies (GWBs), which are essential for the regulation of microRNA (miRNA) and small interfering RNA (siRNA) activity. These intracellular structures serve as critical sites for mRNA storage and degradation, and GWBs integrity is vital for effective gene silencing. Disruption of GWBs can lead to impaired silencing activity, which has been linked to various diseases, including ataxia and Sjögren′s syndrome, where patients develop autoantibodies against GWB proteins. GW182 is ubiquitously expressed and interacts with endogenous argonaute-2 (Ago2), further emphasizing GW182′s importance in the miRNA pathway. Anti-GW182 antibody (E-1) provides a reliable tool for researchers studying the dynamics of GWBs and their implications in cellular processes and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GW182 Antibody (E-1) References:
- The GW182 protein colocalizes with mRNA degradation associated proteins hDcp1 and hLSm4 in cytoplasmic GW bodies. | Eystathioy, T., et al. 2003. RNA. 9: 1171-3. PMID: 13130130
- The RRM domain in GW182 proteins contributes to miRNA-mediated gene silencing. | Eulalio, A., et al. 2009. Nucleic Acids Res. 37: 2974-83. PMID: 19295135
- Optimization of immunoprecipitation-western blot analysis in detecting GW182-associated components of GW/P bodies. | Moser, JJ., et al. 2009. Nat Protoc. 4: 674-85. PMID: 19373232
- Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated gene silencing. | Huntzinger, E., et al. 2010. EMBO J. 29: 4146-60. PMID: 21063388
- Divergent GW182 functional domains in the regulation of translational silencing. | Yao, B., et al. 2011. Nucleic Acids Res. 39: 2534-47. PMID: 21131274
- miRNA-mediated deadenylation is orchestrated by GW182 through two conserved motifs that interact with CCR4-NOT. | Fabian, MR., et al. 2011. Nat Struct Mol Biol. 18: 1211-7. PMID: 21984185
- GW182 proteins cause PABP dissociation from silenced miRNA targets in the absence of deadenylation. | Zekri, L., et al. 2013. EMBO J. 32: 1052-65. PMID: 23463101
- IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression. | Deforzh, E., et al. 2016. Int J Oncol. 49: 2578-2588. PMID: 27840950
- Structural Dynamics of the GW182 Silencing Domain Including its RNA Recognition motif (RRM) Revealed by Hydrogen-Deuterium Exchange Mass Spectrometry. | Cieplak-Rotowska, MK., et al. 2018. J Am Soc Mass Spectrom. 29: 158-173. PMID: 29080206
- RIP-Chip analysis supports different roles for AGO2 and GW182 proteins in recruiting and processing microRNA targets. | Perconti, G., et al. 2019. BMC Bioinformatics. 20: 120. PMID: 30999843
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GW182 Antibody (E-1) | sc-376939 | 200 µg/ml | $322.00 | |||
GW182 Antibody (E-1): m-IgG Fc BP-HRP Bundle | sc-540669 | 200 µg Ab; 10 µg BP | $361.00 | |||
GW182 Antibody (E-1): m-IgG1 BP-HRP Bundle | sc-542285 | 200 µg Ab; 20 µg BP | $361.00 |