GRB10 Double Nickase Plasmid (h): sc-401904-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRB10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GRB10 Double Nickase Plasmid (h) and GRB10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GRB10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GRB10 Antibody (C-11): sc-74509
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRB10 Double Nickase Plasmid (h) | sc-401904-NIC | 20 µg | $410.00 | |||
GRB10 Double Nickase Plasmid (h2) | sc-401904-NIC-2 | 20 µg | $410.00 |
GRB10 (growth factor receptor bound protein 10) is an adaptor protein that binds activated receptor tyrosine kinases, including insulin and IGF-1 receptors, to modulate downstream signaling strength and duration. Through its PH and SH2 domains and proline-rich regions, GRB10 interfaces with PI3K–AKT–mTOR and MAPK pathways and influences receptor trafficking, ubiquitin-dependent turnover, and feedback control of growth factor signaling. These activities connect GRB10 to regulation of cell proliferation, survival, and metabolic homeostasis, with altered GRB10 expression or signaling implicated in cancer-related pathway dysregulation and insulin/IGF axis perturbation in metabolic disease models. GRB10 is also studied in contexts of developmental growth regulation and imprinting-associated phenotypes.
GRB10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GRB10 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GRB10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GRB10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GRB10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.