GPR50 Double Nickase Plasmid (h): sc-402934-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR50 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR50 Double Nickase Plasmid (h) and GPR50 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPR50. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR50 Double Nickase Plasmid (h) | sc-402934-NIC | 20 µg | $410.00 | |||
GPR50 Double Nickase Plasmid (h2) | sc-402934-NIC-2 | 20 µg | $410.00 |
GPR50 encodes an orphan G protein–coupled receptor that is structurally related to melatonin receptors but is considered noncanonical in signaling, with prominent roles in neuroendocrine regulation and circadian-associated biology. It can influence cellular responsiveness to melatonin pathways through receptor–receptor interactions and modulation of GPCR trafficking and signaling competence, impacting downstream cAMP-linked processes and transcriptional programs. Expression patterns and genetic variation in GPR50 have been associated with metabolic phenotypes and neuropsychiatric traits, supporting its relevance in studies of energy balance, mood regulation, and hypothalamic function. As a membrane receptor with tissue-biased expression, GPR50 is also useful for dissecting GPCR network crosstalk and context-dependent signaling outputs in human cellular models.
GPR50 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPR50 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPR50. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPR50 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPR50-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.