GM130 Antibody (4i375): sc-71166

GM130 Antibody (4i375) is a mouse monoclonal IgG2a antibody that detects GM130 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. GM130 plays a crucial role in the organization and function of the Golgi apparatus, serving as a cis-Golgi matrix protein that is essential for the docking of transport vesicles to their target membranes. GM130′s amino-terminus specifically binds to p115, facilitating the formation of a membrane docking site, while GM130′s carboxy-terminus anchors to Golgi membranes. This interaction is vital for vesicle tethering, which is a key step in the transport of proteins from the endoplasmic reticulum to the Golgi compartments. Notably, GM130 undergoes mitotic phosphorylation at Serine 25 by Cdc2, a modification that disrupts binding to p115 and contributes to Golgi fragmentation during mitosis. This phosphorylation occurs in prophase and persists through metaphase and anaphase, with dephosphorylation by PP2A in telophase marking Golgi reassembly. The dynamic regulation of GM130 through phosphorylation highlights GM130′s importance in maintaining Golgi integrity and function during cell division, making anti-GM130 antibody (4i375) an invaluable tool for studying Golgi dynamics and related cellular processes.

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GM130 Antibody (4i375) References:

1. Evidence that Golgi structure depends on a p115 activity that is independent of the vesicle tether components giantin and GM130.  |  Puthenveedu, MA. and Linstedt, AD. 2001. J Cell Biol. 155: 227-38. PMID: 11591729
2. The biogenesis of the Golgi ribbon: the roles of membrane input from the ER and of GM130.  |  Marra, P., et al. 2007. Mol Biol Cell. 18: 1595-608. PMID: 17314401
3. The Golgi protein GM130 regulates centrosome morphology and function.  |  Kodani, A. and Sütterlin, C. 2008. Mol Biol Cell. 19: 745-53. PMID: 18045989
4. Mitotic Golgi vesiculation involves mechanisms independent of Ser25 phosphorylation of GM130.  |  Sundaramoorthy, S., et al. 2010. Cell Cycle. 9: 3100-5. PMID: 20699666
5. GM130 gain-of-function induces cell pathology in a model of lysosomal storage disease.  |  Roy, E., et al. 2012. Hum Mol Genet. 21: 1481-95. PMID: 22156940
6. GM130 Regulates Golgi-Derived Spindle Assembly by Activating TPX2 and Capturing Microtubules.  |  Wei, JH., et al. 2015. Cell. 162: 287-299. PMID: 26165940
7. Structural basis for the interaction between the Golgi reassembly-stacking protein GRASP65 and the Golgi matrix protein GM130.  |  Hu, F., et al. 2015. J Biol Chem. 290: 26373-82. PMID: 26363069
8. Shifted Golgi targeting of glycosyltransferases and α-mannosidase IA from giantin to GM130-GRASP65 results in formation of high mannose N-glycans in aggressive prostate cancer cells.  |  Bhat, G., et al. 2017. Biochim Biophys Acta Gen Subj. 1861: 2891-2901. PMID: 28782625
9. GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation.  |  Giacomello, E., et al. 2019. J Cell Sci. 132: PMID: 30630895
10. The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner.  |  Nakamura, N., et al. 1997. Cell. 89: 445-55. PMID: 9150144

The preferred GM130 antibody is GM130 Antibody (H-7): sc-55590.