GHR CRISPR/Cas9 KO Plasmid (m): sc-420549
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GHR CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GHR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GHR Antibody (B-10): sc-137185
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GHR CRISPR/Cas9 KO Plasmid (m) | sc-420549 | 20 µg | $397.00 |
Overview
Growth hormone receptor (GHR), encoded by the mouse Ghr gene, is a single-pass cytokine receptor that mediates growth hormone signaling to regulate somatic growth, metabolism, and endocrine homeostasis. Ligand-induced receptor dimerization activates JAK2 and downstream STAT5 transcriptional programs, with additional crosstalk to PI3K–AKT and MAPK pathways that influence proliferation, nutrient handling, and survival. GHR-dependent signaling shapes hepatic IGF-1 production and systemic insulin sensitivity, linking receptor activity to growth and metabolic phenotypes. Dysregulated GH–GHR axis function is relevant to research on growth disorders, insulin resistance, and tissue remodeling in endocrine and developmental contexts.
GHR CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ghr gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ghr together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ghr open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GHR protein expression.
This CRISPR knockout system enables efficient generation of Ghr-deficient cell models for investigation of GHR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ghr exon(s) critical for GHR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ghr genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GHR CRISPR/Cas9 KO Plasmid (m) and GHR CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ghr locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GHR HDR Plasmid (m) and GHR HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ghr homology arms to support homology-directed repair at defined Ghr target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.