Date published: 2025-9-8

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GFP Anticorpo (15): sc-101525

4.8(12)
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Fichas de dados
  • GFP Anticorpo 15é um anticorpo monoclonal produzido em camundongo IgG1 κ, citado em 24 publicações, fornecido em 200 µg/ml
  • ativado contra o recombinante GFP
  • recomendado para a detecção de GFP por IF e IHC(P)
  • Atualmente, ainda não concluímos a identificação do(s) reagente(s) de deteção secundário(s) preferido(s) para GFP Anticorpo (15). Este trabalho está em curso.

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VEJA TAMBÉM

A proteína verde fluorescente (GFP) foi originalmente identificada como uma proteína envolvida na bioluminescência da água-viva Aequorea victoria. O cDNA da GFP produz um produto fluorescente quando expresso em células procarióticas, sem a necessidade de substratos ou cofatores exógenos, tornando a GFP uma ferramenta útil para monitorar a expressão gênica e a localização de proteínas in vivo. Vários mutantes de GFP foram desenvolvidos, incluindo o EGFP, que fluoresce mais intensamente do que a GFP selvagem. Seus máximos de excitação deslocados são mais favoráveis para FACs e microscopia de fluorescência, bem como aplicações de marcação dupla. A GFP é amplamente utilizada em vetores de expressão como uma etiqueta de proteína de fusão, permitindo a expressão e o monitoramento de proteínas heterólogas fundidas à GFP.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

GFP Anticorpo (15)

sc-101525
200 µg/ml
$316.00

Is this antibody also recognizing YFP?

Asked by: Adrian31
Thank you for your question,. The antibody does not detect YFP.
Answered by: BlakeJ
Date published: 2023-08-09

You recommended the GFP Antibody (15): sc-101525 for detection of GFP by IHC(P) but I see no picture and cannot find any published data? Could you share your data or help me find published papers. I am OK to test a free sample of the antibody. Thx

Asked by: UCRes
Thank you for your question. We recommend this mouse monoclonal antibody, GFP (15): sc-101525, for detection of GFP by IF and IHC(P) based on publications in the literature. For one reference, please refer to the publication below on PubMed, PMID: 20099318, which is also listed as a product citation on our website (with a link out to PubMed). We are not currently offering a free sample size for this particular antibody. As an alternative, if you would like to test a free sample of GFP Antibody (B-2): sc-9996, please contact our Technical Service Department. This antibody is not currently recommended for IHC(P) because we have not yet tested the antibody with FFPE, however, it also has been published for this application and may be suitable.
Answered by: Technical Support
Date published: 2017-09-11

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with GFP Antibody (15): sc-101525?

Asked by: Trav11
Thank you for your question. We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 3
Date published: 2017-02-03
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Rated 5 out of 5 by from Great antibodyWorked very well with Western blotting and IP. Would highly recommend this antibody.
Date published: 2019-05-10
Rated 4 out of 5 by from GOODI had this one to test the CONSTRUCT, and it worked well in HEK lysate
Date published: 2018-05-14
Rated 5 out of 5 by from Really good antibodyGot this as sample.Now ordering it for future use.
Date published: 2017-12-16
Rated 5 out of 5 by from Good effectGFP used for detect tumor cell, IF of this antibody is obvious.
Date published: 2017-09-06
Rated 5 out of 5 by from Good Antibody!We used this antibody for IF staining and WB, and it has got great results with a nice clear image.
Date published: 2017-09-04
Rated 3 out of 5 by from One work, one didn'tfirst one I bought didn't work. Then the tech person sent me two more, same one I picked still not work. the other one he suggested worked.
Date published: 2017-03-23
Rated 5 out of 5 by from Great IF stainWe used this antibody for IF staining and got great results with a nice clear image
Date published: 2017-01-10
Rated 5 out of 5 by from This antibody is good for Western BlotI used GFP-tag antibody at a dilution of 1:2000 in PBST for 1 h and is enough for an excellent Western blot signal when the protein from DF-1 cell lysate.
Date published: 2016-11-24
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GFP Anticorpo (15) is rated 4.8 out of 5 by 12.
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