GATA3 Double Nickase Plasmid (h): sc-400134-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GATA3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GATA3 Double Nickase Plasmid (h) and GATA3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GATA3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GATA3 Antibody (HG3-31): sc-268
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GATA3 Double Nickase Plasmid (h) | sc-400134-NIC | 20 µg | $410.00 | |||
GATA3 Double Nickase Plasmid (h2) | sc-400134-NIC-2 | 20 µg | $410.00 |
GATA3 encodes a zinc-finger transcription factor that binds GATA motifs to control lineage specification and differentiation programs, with prominent roles in T cell development, Th2 polarization, and epithelial cell fate decisions. In immune cells, GATA3 coordinates cytokine gene expression and chromatin remodeling to shape type 2 inflammatory signaling, while in epithelial contexts it contributes to maintenance of differentiation states through transcriptional network regulation. Perturbation of GATA3-regulated circuits has been linked to immune dysregulation and altered differentiation, making it a widely studied node in transcriptional control, cell identity, and stimulus-responsive gene expression. As a nuclear regulator, GATA3 is frequently interrogated in studies of enhancer usage, transcription factor cooperativity, and cell-state transitions.
GATA3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GATA3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GATA3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GATA3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GATA3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.