gametogenetin Double Nickase Plasmid (m): sc-434015-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- gametogenetin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- gametogenetin Double Nickase Plasmid (m) and gametogenetin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ggn. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
gametogenetin Double Nickase Plasmid (m) | sc-434015-NIC | 20 µg | $410.00 |
Mouse Ggn encodes gametogenetin, a germ cell–enriched protein implicated in spermatogenesis and late-stage gamete differentiation, with prominent expression in testis. Gametogenetin has been linked to nuclear and cytoskeletal organization events that accompany meiotic progression and spermiogenesis, including chromatin remodeling and structural maturation of developing sperm. Disruption of Ggn is therefore relevant to studying pathways that govern germ cell development, genome integrity during meiosis, and fertility-associated phenotypes. These processes intersect with broader reproductive biology and developmental programs that are frequently perturbed in models of male infertility and testicular dysfunction.
gametogenetin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ggn locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ggn. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ggn function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ggn-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.