G_{α o} Antibody (A2): sc-13532

Gα o Antibody (A2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Gα o in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Gα o antibody (A2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gα o plays a crucial role in cellular signaling as a member of the heterotrimeric G protein family, which transmits signals from cell surface receptors to intracellular effectors. Specifically, Gα o is involved in the modulation of ion channels and the regulation of neurotransmitter release, making Gα o essential for processes such as synaptic transmission and muscle contraction. Gα o is primarily located in the plasma membrane, where Gα o interacts with various receptors and effectors, facilitating the conversion of extracellular signals into appropriate cellular responses. Understanding the localization and function of Gα o is vital for elucidating Gα o′s role in physiological processes and implications in various diseases, including neurological disorders and cardiovascular diseases.

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G_{α o} Antibody (A2) References:

1. Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning.  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
2. Acute exercise alters Galphai2 protein expressions through the ubiquitin-proteasome proteolysis pathway in rat adipocytes.  |  Ogasawara, J., et al. 2004. Biochem Biophys Res Commun. 323: 1109-15. PMID: 15381114
3. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
4. Gustducin is a taste-cell-specific G protein closely related to the transducins.  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
5. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
6. Biochemical characterization of three stimulatory GTP-binding proteins. The large and small forms of Gs and the olfactory-specific G-protein, Golf.  |  Jones, DT., et al. 1990. J Biol Chem. 265: 2671-6. PMID: 2105931
7. G protein G(alpha)o is essential for vomeronasal function and aggressive behavior in mice.  |  Chamero, P., et al. 2011. Proc Natl Acad Sci U S A. 108: 12898-903. PMID: 21768373
8. Gαo is a major determinant of cAMP signaling in the pathophysiology of movement disorders.  |  Muntean, BS., et al. 2021. Cell Rep. 34: 108718. PMID: 33535037
9. Cellular Diversity and Differential Subcellular Localization of the G-Protein Gαo Subunit in the Mouse Cerebellum.  |  Roldán-Sastre, A., et al. 2021. Front Neuroanat. 15: 686279. PMID: 34248508
10. Neuron-Restrictive Silencer Factor Limits Myocyte GαO Expression and Is Protective in Heart Failure Progression.  |  Sossalla, S. and Bers, DM. 2022. Circ Res. 130: 249-251. PMID: 35050687
11. G-protein alpha o subunit: mutation of conserved cysteines identifies a subunit contact surface and alters GDP affinity.  |  Thomas, TC., et al. 1993. Proc Natl Acad Sci U S A. 90: 10295-9. PMID: 8234290
12. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{α o} Antibody (A2) has 42 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{α o} Antibody (A2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{α o} Antibody (A2) Specifications

Alternate Product Name: G protein subunit GNAO

Product Clone Name: A2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Gα o

Epitope Species: bovine

Product Reactivity/Detection: G_{α o}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 42 citations in various scientific publications

Available Conjugates/Forms for G_{α o} Antibody (A2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred G_{α o} antibody is G_{α o} Antibody (E-1): sc-393874.