Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FBXO29 CRISPR/Cas9 KO Plasmid (m): sc-433093
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FBXO29 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FBXO29 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FBXO29 Antibody (D-7): sc-514385
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FBXO29 CRISPR/Cas9 KO Plasmid (m) | sc-433093 | 20 µg | $397.00 |
Overview
Fbxw8 encodes the F-box protein FBXO29, a substrate-recognition component of SKP1–CUL1–F-box (SCF) E3 ubiquitin ligase complexes that directs specific proteins for ubiquitination and proteasomal turnover. Through regulated degradation of signaling and cell-cycle regulators, FBXO29 contributes to control of protein homeostasis, proliferation, and differentiation programs. As part of ubiquitin-dependent quality control, SCF-type ligases interface with pathways that shape growth factor signaling and stress responses, making Fbxw8 perturbation relevant for studying mechanisms of dysregulated proteostasis observed in cancer and other proliferative or developmental disorders. Mouse Fbxw8 models are therefore useful for defining ubiquitin-ligase substrate networks and their impact on cellular phenotypes.
FBXO29 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Fbxw8 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Fbxw8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Fbxw8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FBXO29 protein expression.
This CRISPR knockout system enables efficient generation of Fbxw8-deficient cell models for investigation of FBXO29 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Fbxw8 exon(s) critical for FBXO29 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Fbxw8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FBXO29 CRISPR/Cas9 KO Plasmid (m) and FBXO29 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Fbxw8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FBXO29 HDR Plasmid (m) and FBXO29 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Fbxw8 homology arms to support homology-directed repair at defined Fbxw8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.