EYA1 CRISPR Activation Plasmid (h): sc-404056-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EYA1 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- EYA1 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by EYA1 CRISPR Activation Plasmid (h) and EYA1 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the EYA1 transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EYA1 CRISPR Activation Plasmid (h) | sc-404056-ACT | 20 µg | $397.00 |
Human EYA1 (eyes absent homolog 1) encodes a transcriptional coactivator and haloacid dehalogenase–family phosphatase that integrates developmental signaling with gene regulatory programs. EYA1 functions in concert with SIX and DACH proteins to control organogenesis and cell fate decisions, and it also participates in DNA damage responses through modulation of phosphorylation-dependent signaling. Dysregulated EYA1 activity or expression is linked to congenital developmental disorders affecting ear and kidney morphogenesis and has been investigated in contexts where altered differentiation and proliferation programs contribute to disease phenotypes. As a nuclear-cytoplasmic regulator, EYA1 provides a tractable node for studying transcriptional control, phosphatase biology, and developmental pathway crosstalk.
EYA1 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous EYA1 expression without altering the underlying DNA sequence.
EYA1 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the EYA1 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the EYA1 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous EYA1 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native EYA1 locus and enabling the study of EYA1-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of EYA1 pathway restoration in tumor cells with silenced or reduced EYA1 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.