Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ETBR Double Nickase Plasmid (h): sc-401804-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ETBR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ETBR Double Nickase Plasmid (h) and ETBR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EDNRB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ETBR Antibody (5H2): sc-293198
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ETBR Double Nickase Plasmid (h) | sc-401804-NIC | 20 µg | $410.00 | |||
ETBR Double Nickase Plasmid (h2) | sc-401804-NIC-2 | 20 µg | $410.00 |
EDNRB encodes endothelin receptor type B (ETBR), a G protein–coupled receptor that binds endothelin peptides to regulate intracellular calcium flux, phospholipase C signaling, and downstream MAPK/ERK and PI3K-associated pathways. ETBR activity contributes to neural crest cell migration and differentiation, melanocyte development, and vascular tone control through endothelin-dependent signaling networks. In human biology, EDNRB dysfunction is linked to disorders of enteric nervous system development such as Hirschsprung disease and to pigmentation phenotypes, reflecting its role in developmental patterning and cell motility programs. Altered EDNRB signaling is also studied in the context of tumor cell–microenvironment interactions and lineage plasticity, making it relevant for mechanistic studies of GPCR signaling and developmental pathways.
ETBR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EDNRB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EDNRB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EDNRB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EDNRB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.