ErbB4/HER4 Double Nickase Plasmid (h): sc-400275-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ErbB4/HER4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ErbB4/HER4 Double Nickase Plasmid (h) and ErbB4/HER4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ERBB4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ErbB4/HER4 Antibody (C-7): sc-8050
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ErbB4/HER4 Double Nickase Plasmid (h) | sc-400275-NIC | 20 µg | $410.00 | |||
ErbB4/HER4 Double Nickase Plasmid (h2) | sc-400275-NIC-2 | 20 µg | $410.00 |
ERBB4 encodes ErbB4/HER4, a ligand-activated receptor tyrosine kinase in the EGFR/ErbB family that transduces signals from neuregulins and related growth factors. Upon activation, ErbB4 engages MAPK/ERK and PI3K–AKT signaling, and can participate in JAK/STAT-dependent transcriptional programs, influencing proliferation, differentiation, and survival in a context-dependent manner. ERBB4 also supports cell–cell communication and developmental processes in neural and cardiac tissues through heterodimerization with other ErbB receptors and regulated receptor processing. Dysregulated ERBB4 expression, mutation, or signaling balance is implicated in oncogenic pathway rewiring and neurodevelopmental phenotypes, making it a useful target for mechanistic studies of receptor signaling networks.
ErbB4/HER4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ERBB4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ERBB4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ERBB4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ERBB4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.