Eps15 Double Nickase Plasmid (h): sc-402303-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Eps15 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Eps15 Double Nickase Plasmid (h) and Eps15 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EPS15. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Eps15 Antibody (E-3): sc-390259
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Eps15 Double Nickase Plasmid (h) | sc-402303-NIC | 20 µg | $410.00 | |||
Eps15 Double Nickase Plasmid (h2) | sc-402303-NIC-2 | 20 µg | $410.00 |
EPS15 encodes epidermal growth factor receptor substrate 15 (Eps15), an endocytic adaptor that localizes to clathrin-coated pits and coordinates receptor internalization. Eps15 interacts with AP-2, clathrin, and ubiquitin-binding partners to regulate cargo selection, vesicle formation, and trafficking decisions that shape downstream signaling. Through these roles, EPS15 influences growth factor receptor turnover and signal attenuation within endocytic and ubiquitin-dependent pathways. Dysregulation of endocytosis and receptor trafficking involving EPS15 has been studied in contexts such as altered proliferative signaling and perturbations of vesicular transport programs relevant to disease biology.
Eps15 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EPS15 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EPS15. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EPS15 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EPS15-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.