Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EphB2 Double Nickase Plasmid (h): sc-401642-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EphB2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EphB2 Double Nickase Plasmid (h) and EphB2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EPHB2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EphB2 Antibody (2D12C6): sc-130068
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EphB2 Double Nickase Plasmid (h) | sc-401642-NIC | 20 µg | $410.00 | |||
EphB2 Double Nickase Plasmid (h2) | sc-401642-NIC-2 | 20 µg | $410.00 |
EPHB2 encodes EphB2, a receptor tyrosine kinase of the Eph family that binds membrane-tethered ephrin-B ligands to drive contact-dependent signaling. EphB2 regulates cell positioning, axon guidance, and tissue boundary formation through bidirectional signaling that remodels the actin cytoskeleton and modulates adhesion via pathways involving Rho-family GTPases, MAPK/ERK, PI3K, and Src-family kinases. In epithelial and neural contexts, EphB2 contributes to cell migration and compartmentalization, and altered EphB2 signaling has been linked to dysregulated growth and invasion phenotypes in cancer models. EphB2 is also implicated in neurodevelopmental processes and synaptic organization, making it a useful node for studying signaling crosstalk that shapes cell fate and connectivity.
EphB2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EPHB2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EPHB2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EPHB2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EPHB2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.